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Code
OSC00155G
$240USD    Buy Now 

ID Tag
OSX12-210309

Immunogen
A synthetic peptide from human Cathelicidin antimicrobial peptide (LL-37) conjugated to blue carrier protein has been used as the antigen.
The location of the epitope is highlighted in green.

Accession

Also known
LL37, LL-37

Target
Cathelicidin antimicrobial protein is an antimicrobial protein found in specific granules of polymorphonuclear leukocytes.
FUNCTION: Binds to bacterial lipopolysaccharides (LPS), has antibacterial activity.
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Expressed in bone marrow and testis and neutrophils.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Agerberth B, et al. Proc. Natl. Acad. Sci. U.S.A. 92:195-199(1995).
2. Cowland J.B, et al. FEBS Lett. 368:173-176(1995).
3. Larrick J.W, et al. Infect. Immun. 63:1291-1297(1995).
4. Larrick J.W, et al. FEBS Lett. 398:74-80(1996).

Limitation
For research use only

Related
products

Code
OSC00155G
$240USD    Buy Now 

Unit size
100 ug

Conjugate
Unconjugated antibody

Host
Mouse

Purity
IgG

Clonality
Clone OSX12, monoclonal

Isotype
IgG1,k

Applications
IHC, IF, WB, flow cytometry. Use at a concentration of 5-10 ug/ml. The optimal concentration should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Cathelicidin antimicrobial peptide.

Spcs X-react.
Human, other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 ul of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSC00155G
$240USD    Buy Now 


Mouse monoclonal to Cathelicidin: OSX12 clone IF on ethanol-fixed human spermatosoid using Mouse monoclonal to Cathelicidin: OSX12 clone (OSC00155G) at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.
Mouse monoclonal to Cathelicidin: OSX12 clone IF on ethanol-fixed human spermatosoid using Mouse monoclonal to Cathelicidin: OSX12 clone (OSC00155G) at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.
Mouse monoclonal to Cathelicidin: OSX12 clone IF on ethanol-fixed human spermatosoid using Mouse monoclonal to Cathelicidin: OSX12 clone (OSC00155G) at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.
Mouse monoclonal to Cathelicidin: OSX12 clone IF on ethanol-fixed human spermatosoid using Mouse monoclonal to Cathelicidin: OSX12 clone (OSC00155G) at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.
Mouse monoclonal to Cathelicidin: OSX12 clone IF on ethanol-fixed human spermatosoid using Mouse monoclonal to Cathelicidin: OSX12 clone (OSC00155G) at a concentration of 10 ug/ml. DAPI counterstained appearing in blue.
Mouse monoclonal to Cathelicidin: OSX12 clone Staining of a cytospin preparation of peripheral blood mononuclear cells (PBMC) isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Mouse monoclonal to Cathelicidin: OSX12 clone (OSC00155G) at a concentration of 10 ug/ml. DAPI counterstained appearing in blue. The antibody selectively recognizes polymorphonuclar cells.
Mouse monoclonal to Cathelicidin: OSX12 clone Staining of a cytospin preparation of peripheral blood mononuclear cells (PBMC) isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Mouse monoclonal to Cathelicidin: OSX12 clone (OSC00155G) at a concentration of 10 ug/ml. The antibody selectively recognizes polymorphonuclar cells.
Mouse monoclonal to Cathelicidin: OSX12 clone Staining of a cytospin preparation of peripheral blood mononuclear cells (PBMC) isolated from buffycoat. Cells were left to air dry and then fixed with cold acetone (90 seconds) and blocked with PBS containing 1% FCS and 0.1% saponin (blocking buffer) for 20 minutes. Cells were then washed twice in PBS and incubated with Mouse monoclonal to Cathelicidin: OSX12 clone (OSC00155G) at a concentration of 10 µg/ml. DAPI counterstained appearing in blue. The antibody selectively recognizes polymorphonuclar cells.
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Mouse monoclonal to Cathelicidin: OSX12 clone
Content

Download Protocols

APES coating of slides
Loading Buffer: Reducing
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
....G6 Lysing Buffer
Content

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