A synthetic peptide from the cytoplasmic domain of human STIM1 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
FUNCTION: Plays a role in mediating Ca2+ influx following depletion of intracellular Ca2+ stores. Acts as Ca2+ sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca2+ depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca2+ release-activated Ca2+ (CRAC) channel subunit, TMEM142A/ORAI1.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein. Note: Translocates from the endoplasmic reticulum to the cell membrane in response to a depletion of intracellular calcium.
Tissue specificity: Ubiquitously expressed in various human primary cells and tumor cell lines.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Parker N.J, et al. Genomics 37:253-256(1996)
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1 : 1000 is recommended for WB and IHC-P. The optimal dilution should be determined by the end user. Not tested in other applications.
Specific for STIM1.
Human, rat, mouse, marmoset. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.