⚬ Services
Code
OSP00050W
$240USD    Buy Now 

ID Tag
Rb755-140209-WS

Immunogen
A synthetic peptide from the c-terminal region of human Pr3 conjugated to blue carrier protein was used as the antigen.

Accession

Also known
Leukocyte proteinase 3, AGP7, Myeloblastin, Wegener autoantigen, PR-3, P29, C-ANCA antigen, Neutrophil proteinase 4, MBN, PRTN3, NP-4

Target
FUNCTION: Polymorphonuclear leukocyte serine protease that degrades elastin, fibronectin, laminin, vitronectin, and collagen types I, III, and IV (in vitro) and causes emphysema when administered by tracheal insufflation to hamsters.
Catalytic activity: Hydrolysis of proteins, including elastin, by preferential cleavage: -Ala-|-Xaa- > -Val-|-Xaa-.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Labbaye C, et al. Proc. Natl. Acad. Sci. U.S.A. 88:9253-9256(1991)
2. Grimwood J, et al. Nature 428:529-535(2004)

Limitation
For research use only

Related
products

Code
OSP00050W
$240USD    Buy Now 

Unit size
100 µl

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
WB, IHC, flow cytometry. A dilution of 1: 200 to 1: 1000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.

Specificity
Specific for Pr3.

Spcs X-react.
Human. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.

Note
Control peptide is available at $120 per 50 μg. Please enquire sales@osenses.com

Code
OSP00050W
$240USD    Buy Now 


Rabbit antibody to Pr3 WB on human PMN (peripheral blood mononuclear cells isolated from buffycoat; denatured, reduced) using Rabbit antibody to Pr3 (OSP00050W) at 1: 500 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.
Rabbit antibody to Pr3 Human PBMC were isolated and adjusted to 106 cells mL-1. Cells were fixed with 2% formaldehyde for 10 minutes at 37°C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 minutes at room temperature (RT). Excess of blocking solution was removed and cells were then incubated with Rabbit antibody to Pr3 (OSP00050W) for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 minutes. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum (OSP00050W).
Rabbit antibody to Pr3 Human PBMC were isolated and adjusted to 106 cells mL-1. Cells were fixed with 2% formaldehyde for 10 minutes at 37°C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 minutes at room temperature (RT). Excess of blocking solution was removed and cells were then incubated with Rabbit antibody to Pr3 (OSP00050W) for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 minutes. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum (OSP00050W).
Rabbit antibody to Pr3 Human PBMC were isolated and adjusted to 106 cells mL-1. Cells were fixed with 2% formaldehyde for 10 minutes at 37°C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 minutes at room temperature (RT). Excess of blocking solution was removed and cells were then incubated with Rabbit antibody to Pr3 (OSP00050W) for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 minutes. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum (OSP00050W).
Rabbit antibody to Pr3 Human PBMC were isolated and adjusted to 106 cells mL-1. Cells were fixed with 2% formaldehyde for 10 minutes at 37°C. Washed twice with PBS before cytospin the cells onto microscope slides. Cells were blocked with PBS containing 1%BSA for 20 minutes at room temperature (RT). Excess of blocking solution was removed and cells were then incubated with Rabbit antibody to Pr3 (OSP00050W) for 30 min at RT (diluted 1:100 in the blocking buffer). Washed 3X with PBS and incubated with anti-Rabbit Alexa 586 for further 30 minutes. Washed as before and nuclear counterstained with DAPI. Neutrophils and Monocytes, known to have PR3 are intensely stained by the Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum (OSP00050W).
⚬ Products
Rabbit antibody to Pr3
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

Watch Videos

Autoperfuser™ OS2100
Red Carpet: A Histology Carpet for Slides