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Code
OST00198W
$240USD    Buy Now 

ID Tag
Rb619-170808-WS

Immunogen
A synthetic peptide from rat TRPC3 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and human.
The location of the epitope is highlighted in green.

Accession

Also known
Short transient receptor potential channel 3, transient receptor potential cation channel, subfamily C, member 3

Target
FUNCTION: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol-1,4,5-triphosphate receptors (ITPR) with bound IP3. May also be activated by internal calcium store depletion.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.

Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20C for long term storage and refrigerated at 2-8C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

References
1. Ohki G, et al. J. Biol. Chem. 275:39055-39060(2000).

Limitation
For research use only

Code
OST00198W
$240USD    Buy Now 

Unit size
100 l

Conjugate
Unconjugated antibody

Host
NZ white rabbit

Purity
Whole serum

Clonality
Polyclonal

Isotype
Polyclonal, whole serum

Applications
IHC, WB. A dilution of 1 : 1000 is recommended for WB and 1:500 for IF. The optimal dilution should be determined by the end user.

Specificity
Specific for TRPC3.

Spcs X-react.
Rat, mouse, human, marmoset. Other species not yet tested.

Format
Lyophilised

Reconstitution
Reconstitute in 100 l of sterile water. Centrifuge to remove any insoluble material.

Code
GST00032P
$120USD   

ID Tag
53756-6

Unit size
50 g

Accession

Also known
Short transient receptor potential channel 3, transient receptor potential cation channel, subfamily C, member 3

Target
This is the immunising peptide for the product Rabbit antibody to 4th cytoplasmic loop of TRPC3: whole serum (OST00198W)

Purity
>82%

Applications
Control peptide

Format
Lyophilised

Reconstitution
This peptide is soluble in PBS and water.

Storage
Maintain refrigerated at 2-8C for up to 12 months. After reconstitution keep at -20C. Avoid freeze and thaw cycles.

Expiry Date
12 months after reconstitution

Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.

Limitation
For research use only

Related Products
OST00198W

Code
OST00198W
$240USD    Buy Now 


Rabbit antibody to TRPC3 IF on frozen sections of mouse brain. Primary antibody dilution 1: 500.
Rabbit antibody to TRPC3 IF on frozen sections of mouse brain. Primary antibody dilution 1: 50
Rabbit antibody to TRPC3 IF on frozen sections of mouse brain. Primary antibody dilution 1: 50
Rabbit antibody to TRPC3 IF on frozen sections of mouse brain. Primary antibody dilution 1: 50
Rabbit antibody to TRPC3 IF on frozen sections of mouse brain. Primary antibody dilution 1: 50
Rabbit antibody to TRPC3 WB on tissue lysates. Note the profound effect of composition of the lysing buffer on WB. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
Rabbit antibody to TRPC3 WB on mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details.
⚬ Products
Rabbit antibody to TRPC3
Content

Download Protocols

APES coating of slides
SDS Loading Buffer
IHC-P on Autostainer
G1 Lysing Buffer
WB Protocol online
IHC-P HIER (Tris-EDTA, pH 9)
4% PFA
Davidson's fix (modified)
....Tissue lysate perparation
....G2 Lysing Buffer
....R Lysing Buffer
....G3 Lysing Buffer
....G4 Lysing Buffer
....G5 Lysing Buffer
.G6 Lysing Buffer
Content

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