A synthetic peptide from rat TRPC3 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and human.
The location of the epitope is highlighted in
Short transient receptor potential channel 3, transient receptor potential cation channel, subfamily C, member 3
FUNCTION: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol-1,4,5-triphosphate receptors (ITPR) with bound IP3. May also be activated by internal calcium store depletion.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
12 months after reconstitution
This item will be shipped to you at ambient temperature in a lyophilised form.
1. Ohki G, et al. J. Biol. Chem. 275:39055-39060(2000).
For research use only
NZ white rabbit
Polyclonal, whole serum
IHC, WB. A dilution of 1 : 1000 is recommended for WB and 1:500 for IF. The optimal dilution should be determined by the end user.
Specific for TRPC3.
Rat, mouse, human, marmoset. Other species not yet tested.
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.