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Code
OST00198W
$240USD
ID Tag
Rb619-170808-WS
Immunogen
A synthetic peptide from rat TRPC3 conjugated to blue carrier protein was used as the antigen. The peptide is homologous in mouse and human.
The location of the epitope is highlighted in green.
The location of the epitope is highlighted in green.
Accession
Also known
Short transient receptor potential channel 3, transient receptor potential cation channel, subfamily C, member 3
Target
FUNCTION: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C, and by inositol-1,4,5-triphosphate receptors (ITPR) with bound IP3. May also be activated by internal calcium store depletion.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
Storage
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
Expiry Date
12 months after reconstitution
Shipping
This item will be shipped to you at ambient temperature in a lyophilised form.
References
1. Ohki G, et al. J. Biol. Chem. 275:39055-39060(2000).
Limitation
For research use only
Code
OST00198W
$240USD
Unit size
100 µl
Conjugate
Unconjugated antibody
Host
NZ white rabbit
Purity
Whole serum
Clonality
Polyclonal
Isotype
Polyclonal, whole serum
Applications
IHC, WB. A dilution of 1 : 1000 is recommended for WB and 1:500 for IF. The optimal dilution should be determined by the end user.
Specificity
Specific for TRPC3.
Spcs X-react.
Rat, mouse, human, marmoset. Other species not yet tested.
Format
Lyophilised
Reconstitution
Reconstitute in 100 µl of sterile water. Centrifuge to remove any insoluble material.
- Click to see the eLab Book
Code
OST00198W
$240USD
PDF Data Sheet
- Click to download the Data SheetMSDS
Code
OST00198W
$240USD
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IF on frozen sections of mouse brain. Primary antibody dilution 1: 500. |
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IF on frozen sections of mouse brain. Primary antibody dilution 1: 50 |
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IF on frozen sections of mouse brain. Primary antibody dilution 1: 50 |
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IF on frozen sections of mouse brain. Primary antibody dilution 1: 50 |
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IF on frozen sections of mouse brain. Primary antibody dilution 1: 50 |
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WB on tissue lysates. Note the profound effect of composition of the lysing buffer on WB. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details. |
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WB on mouse tissue lysates. Blocking: 1% LFDM for 30 min at RT; primary antibody: dilution 1:1000 incubated at 4C overnight. Click on eLAB BOOK tab to see the details. |
⚬ Products
Rabbit antibody to TRPC3
